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. 2018 May 8;8:7143. doi: 10.1038/s41598-018-25315-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Responses of SmDML genes to 5Aza-C treatment. Fold changes of SmDMLs in leaves of S. miltiorrhiza plantlets treated with 1, 5, 10, 30 or 50 µM of 5Aza-C for 15 days are shown. The expression levels were analyzed using the quantitative RT-PCR method. Expression level in leaves without treatment (0 µM) was arbitrarily set to 1 and the levels in leaves of 5Aza-C-treated plantlets were given relative to this. One-way ANOVA was calculated using IBM SPSS 20 software. P < 0.05 was considered statistically significant and was represented by asterisks.