Figure 10.

Macropinocytosis – stimulating agents re-direct virus trafficking in the cell. HCT-8 cells pre-treated with caveolin-1 and dynamin inhibitors were infected with HCoV-OC43 in presence or absence of dextran and viral yield was assessed by RT-qPCR 5 days p.i. nys −10 μg/ml nystatin; MM −10 μM MiTMAB; NH₄Cl −50 mM NH₄Cl; V – non-treated HCoV-OC43 infected cells; dex – dextran-TMR. The data is presented as the mean of a triplicate for each sample ± SD. To determine the significance of differences between compared groups, Single-Factor Analysis of Variance (ANOVA) was applied. ***P values < 0.05 were considered significant; ns – not significant.