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. 2018 May 8;8:7262. doi: 10.1038/s41598-018-25435-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Perlecan Domain IV-3 mediates a strong repulsive/clustering effect on C4-2 cells as does full length perlecan. (A) Schematic of full length perlecan highlighting Domain IV-3 (the last 7 Ig repeats of Domain IV). A reverse phase protein array (RPPA) was performed on cells cultured on wells entirely coated with Domain IV-3 (panel B) or MMP-7 cleaved Domain IV-3 (panel C) for 48 hours. Cells cluster on Domain IV-3 and this is reversed by pre-cleavage with MMP-7. D.) C4-2 cells 72 hours after being cultured on a spot (inside the white dashed circle) of surface adsorbed Domain IV-3. Cells cleared out of the area, creating a border around Domain IV-3. Scalebars are 250 µm. White asterisks indicate fiduciary markers (black areas) used to approximate the location of the spotted Domain IV-3 at the time.