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. 2018 May 8;8:7256. doi: 10.1038/s41598-018-24999-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Subcellular localization of transiently expressed MiISE5^Δsp:GFP in Arabidopsis protoplasts and testing of transcriptional activation ability of MiISE5 in yeast. (A) MiISE5^Δsp was localized to the cytoplasm. (B) The MiISE5 was fused with GAL4BD and expressed in yeast cells. GAL4BD and GAL4BD-GAL4AD were used as negative and positive controls, respectively. The blue yeast colonies on synthetic medium without tryptophan (SC/-Trp) with the chromogenic substrate 5-bromo-4-chloro-3-indolyl-αomogalactopyranoside (X-α-Gal) indicate the expression of MEL-1 gene. The successful growth of yeast colonies on synthetic medium without tryptophan and histidine (SC/-Trp/-His) indicate the expression of the HIS3 reporter gene. Scale bar = 50 μm.