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. 2018 May 8;8:7264. doi: 10.1038/s41598-018-24203-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Decreased expression of miR-125b and miR-10a is associated with increased lineage commitment of myeloid progenitors. (a) qRT-PCR validation of miRNAs differentially expressed in TLDA experiments (n = 10). The small nucleolar RNA RNU24 was used for normalisation. Whiskers show 5th and 95th percentiles. miR-135a could not be amplified from most monocyte and neutrophil samples; the results have therefore been shown here as expression relative to RNU24 only. Differences were tested by ANOVA, Kruskal Wallis test with Dunn’s post test. ***p < 0.001, **p < 0.01, *p < 0.05, n.s. = not significant. (b) Expression of CD34, CD11b, CD14 and CD15 on MACS-purified cord blood CD34⁺ cells cultured for 6 days with SCF (100 ng/mL) and GCSF (10 ng/mL). (c) The same cytokines were used to in vitro differentiate FACS-purified CD34⁺ cells and their levels of miR-223, miR-125b and miR-10a assessed by q-RT-PCR over nine days (n = 3). Graphs show mean ± SEM.