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. 2018 May 8;8:7136. doi: 10.1038/s41598-018-25609-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunization with amphipol-BamA and Ribi adjuvant generates robust antibody response. (a) Anti-BamA pAb titers using purified pAbs were measured by ELISA for BamA immunized mice with either Ribi (n = 5; solid lines) or CFA (n = 4; dashed lines) adjuvants. (b) Mean fluorescent intensity (MFI) as determined by flow cytometry of 40 μg/mL BamA pAbs against ΔwaaD (black box) or K12 strains (white box). M1-M5 represent individual mice from the Ribi immunized group. MFI for irrelevant antibody control is depicted by red dashed line. (c) MFI values for individual mice from either the Ribi adjuvant group (black box) or CFA adjuvant group (grey box). (d) Summary of ELISA and FACS results for panel of hybridomas from mice immunized with detergent-solubilized or amphipol-BamA.