Table 2.

Biological classification based on gene ontology annotation of proteins identified by mass spectrometry analysis of immunoaffinity-purified plasma exosomes.

Biological classification	HIV-negative (n = 3)	HIV-positive (n = 4)
immune activation/inflammation	ADAM33, AZGP1, BPIFA1, BPIFB1, BTN2A2, CAMP, CD4, CSF1R, CST6, CXCL16, DDR1, DMBT1, ENO1, FERMT3, LILRB1, LILRB2, LYZ, PLTP, TFRC	ADAM33, BPIFB1, BTN2A2, CAMP, CD14, COLEC10, CPN1, CRP, CSF1R, DDR1, ENO1, FERMT3, HLA-A, HLA-B, ITGB1, ICAM2, LILRB1, PLTP, TFRC
transmembrane signaling	CD4, EFNA4, LRP8, NOTCH4, PTRF, TFRC	EFNA4, ITGA6, LRP8, NOTCH4, TFRC
extracellular vesicles/exosomes	ANXA2, APMAP, AZGP1, BPIFB1, CAMP, CAT, CD9, CD63, CD81, CDH1, ENO1, FERMT3, FLNA, GAPDH, HSPA5, LAMP1, LDHA, MYH9, PFN1, PKM, PRDX1, PRDX2, PXDN, RAP1A, SDCBP, TXN, UBA52, YWHAB, ZG16B	ADAM10, APMAP, ARF1, BPIFB1, CAMP, CDH1, CDC42, CRTAC1, ENO1, FERMT3, GAPDH, GPLD1, LAMP1, MYH9, PCYOX1, PKM, RAB1A, RAC1, RAP1A, SDCBP, SPTAN1, STOM, TLN1, UBA52
stress response	CAT, CDH1, ENO1, GAPDH, HSPA5, HSPA1L, PRDX1, PRDX2, TXN, TYMP, WARS	ADAM10, BMP1, CDH1, ENO1, GAPDH, MAP3K11, RAC1, SEPP1
oxidative stress	CAT, ENO1, GAPDH, PRDX1, PRDX2, PXDN, TPM1, TXN, WARS	CRP, ENO1, GAPDH, GPX3, ITGB1, RAC1, SLC25A33, SEPP1
fatty acid/lipid metabolism	AZGP1, CD36, CETP, DPEP3, FABP5, HGFAC, LRCOL1, PKM, PLTP	ACOX3, ADIPOQ, ANGPTL3, CD36, CETP, DPEP3, GPLD1, HADHA, HGFAC, LRCOL1, PCSK9, PKM, PLTP, SAR1A
platelets	CD36, FERMT3, ITGA2B, ITGB3, MMRN1	CD36, FERMT3, F11R, ITGA2B, ITGB3, PF4V1

PANTHER and Biobase TRANSFAC tools were used for gene ontology (GO) mapping of proteins identified by mass spectrometry analysis of IAP-purified plasma exosomes from 3 HIV-negative and 4 HIV-positive subjects in the merged datasets shown in Supplemental Tables 3 and 4. GO groups were assigned to the indicated biological functions. Individual proteins may be annotated to more than one category. Proteins identified by 2 or more unique peptides are shown in bold.