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. 2018 May 8;8:7297. doi: 10.1038/s41598-018-25453-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Aβ42 selectively induces microglial mature BDNF expression and release. MTT analysis of Aβ42 (0.5 μM) toxicity on differentiated SH-SY5Y cells treated for different time points, as indicated (A). Western blot analysis of BDNF expression in pMG (B) or pAS (C) treated with Aβ42 (0.5 μM) for 5 h or pulsed for 5 h followed by medium change and further incubation without Aβ for 18 h (labeled as 5 + 18 h). Western blot analysis of BDNF expression in SH-SY5Y cells exposed to Aβ42 (0.5 μM) for 5 h (D). ELISA determination of released BDNF in medium collected from pMG treated for 5 h or 5 + 18 h (E). Image cytofluorometric analysis of co-localization of BDNF with integrin α-M (int; F) or GFAP (G) in mxG cells. BF = brightfield. Double immunostaining of BDNF (green) and integrin α-M (red) in control vs Aβ42-treated (0.5 μM for 5 h) pMG (H). Scale bar is 20 μm. Blots were cropped to display specific bands. Original blots are reported in Supplementary Fig. 1. Values are mean ± SEM with n = 3-5. *p < 0.05 vs. untreated control by one-way ANOVA followed by Newman-Keuls test for significance or Student’s t-test, as appropriate.