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. 2018 May 8;8:7297. doi: 10.1038/s41598-018-25453-1

Figure 3.

Figure 3

CM from microglia prevents Aβ42-driven SYP loss. Western blot analysis of SYP in SH-SY5Y cells exposed for 5 h to Aβ42 (0.5 μM) in different conditions. In A, cells were treated with Aβ42 alone or in combination with BDNF in non-conditioned medium (A). In (B), cells were exposed to Aβ42 alone or in combination with GNF5837 (GNF) in CM from untreated pMG (CMC) or from pMG treated with Aβ according to the 5 + 18 h-pulse protocol (CMA). Cells were treated with Aβ42 alone or in combination with ANA-12 (20 μM; C) or U0126 (10 μM; D) in the presence of CMA. Cells were treated with Aβ42 alone or in combination with GNF (100 nM) in CM from BDNF (1 ng/ml)-treated pMG (CMB; E) or CM from Aβ42 + GNF-treated pMG (CMAG; F). Blots were cropped to display specific bands. Original blots are reported in Supplementary Fig. 2. Values are mean ± SEM with n = 3–4. *p < 0.05 vs. respective controls by one-way ANOVA followed by Newman-Keuls test for significance or Student’s t-test, as appropriate.