Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 May 8;8:7297. doi: 10.1038/s41598-018-25453-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

CM from microglia restores synaptic activity impaired following Aβ42 exposure. SH-SY5Y cells were treated for 5 h with Aβ42 (0.5 μM) in non-conditioned medium or in CM from untreated (CMC) or Aβ-treated (CMA) pMG. Cells were stained with FM 1–43 dye and treated to allow formation of labeled synaptic vesicles (loading). Fluorescence intensity was evaluated at the end of the loading step (A) and after unloading of fluorescent vesicles by stimulation of exocytosis (B). Representative images of cells stained with FM 1–43 in each condition, in the loading and unloading step, are reported next to corresponding graphs. Immunostaining of SYP (red) in SH-SY5Y cells exposed to Aβ42 (0.5 μM) for 5 h in CMA (C). Nuclei are stained with DAPI (blue). Values are mean ± SEM with n = 3–5. Scale bar is 10 μm. *p < 0.05 vs. respective controls by one-way ANOVA followed by Newman-Keuls test for significance.