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. 2018 May 8;8:7296. doi: 10.1038/s41598-018-25480-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Heparin-treated macrophages have increased ferroportin levels. (A) Ferroportin expression in heparin-treated macrophages 48 hours after BCG infection (63X). (B) Ferroportin expression in heparin-treated macrophages 48 hours after BCG infection, measured as Pixel MFI/cell from a minimum of 20 cells in three different fields (40X) from three independent experiments. (C) Percentage of BCG bacilli colocalized with ferroportin-FITC staining (overlapping red and green pixels). Data from three independent experiments. **p < 0.01, ***p < 0.001.