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. 2018 May 8;8:7296. doi: 10.1038/s41598-018-25480-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Heparin decreases iron availability to intracellular mycobacterial bacilli. (A) Ferritin expression in heparin-treated macrophages 48 hours after BCG infection. (B) Percentage of ferritin -positive BCG bacilli in five fields from three independent experiments as represented in (A). (C) Nuclear ferritin in heparin-treated macrophages 48 hours after BCG infection measured by percent of double-positive pixels (FITC and DAPI). (D) Total intracellular iron staining (Prussian blue) in heparin-treated macrophages 48 hours after BCG infection. Data from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001.