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. 2018 May 8;8:7174. doi: 10.1038/s41598-018-25381-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of SE-EE on scopolamine inflicted neuroinflammation in C57/BL6N mice: (a) iNOS and COX-2 protein expressions in the hippocampus (n = 3) was determined by western blotting analysis (respective markers were acquired by cropping from the same gel, full length blots are in Supplementary data- S.3.2) and (b,c) represents the quantification of inflammatory protein expression relative to β-actin, which was achieved using the ImageJ software; (d,e,f and g) represent the level of TNF-α, IL-1β, IL-6 and IL-10 proteins in the hippocampus (n = 5, pooled biological replicates), respectively, as acquired via ELISA. Data are expressed as mean ± SD. One-way ANOVA-Tukey’s multiple comparison test was performed. ^#p < 0.05 compared with the control group; *p < 0.05 other treated groups compared with the scopolamine group.