Fig. 3.

The gene transcript (A) and protein expression (B) levels of energy metabolism related factors (AMPKα, SIRT1 and PGC-1α) regulated by dietary BCAAs in DSA and ASA tissues of the growing pigs. We used β-actin as an internal control. Data were represented as mean ± SE (n = 6); Values with different letters (a, b, c) are considered as significant difference (P < 0.05). DSA = dorsal subcutaneous adipose; ASA = abdominal subcutaneous adipose; AP = adequate protein diet; LP = low protein diet; LP + B = the LP diet supplemented with BCAAs; LP + 2B = the LP diet supplemented with two times amount of BCAAs.