Table 2.
Primers used for real-time PCR analysis.
| Target genes | Primer sequences (5′–3′) | Products, bp | Genbank accession No. |
|---|---|---|---|
| LAT1/SLC7A7 | F: TTTGTTATGCGGAACTGG | 155 | NM_001110421 |
| R: AAAGGTGATGGCAATGAC | |||
| LAT4/SLC43A2 | F: ACGGAGCAAGTAACCCCAGC | 235 | XM_003358191 |
| R: GCCACGAGGATGACGATGAA | |||
| SNAT2/SLC38A2 | F: TACTTGGTTCTGCTGGTGTCC | 212 | XM_003126626 |
| R: GTTGTGGGCTGTGTAAAGGTG | |||
| AMPKα | F: CAGACAGCCCTAAAGCAAGA | 311 | NM_214266 |
| R: CTCCAGCACCTCATCATCAA | |||
| PGC-1α | F: GCCCAGTCTGCGGCTATTT | 265 | GU991077 |
| R: GTTCAGCTCGGCTCGGATTT | |||
| SIRT1 | F: GGTTTGAAGAATGTTGCCTG | 114 | NM_001145750 |
| R: CCGTTTACTAATCTGCTCCT | |||
| Cyt c | F: CTGCGAGTGGTGGATTGT | 222 | NM_001129970 |
| R: ATGCCTTTGTTCTTGTTGG | |||
| ATPase 6 | F: CTATTCCCAACACCCAAACG | 196 | AJN90987 |
| R: TGGGTGTGAATGAGTGTGGT | |||
| UCP2 | F: CACCAAGGGCTCTGAGCATG | 387 | XM_005667098 |
| R: TCTACAGGGGAGGCGATGAC | |||
| UCP3 | F: GACGTGGTGAAGGTTCGATT | 330 | DQ530368 |
| R: CGAGTTCATGTACCGGGTCT | |||
| NRF-1 | F: TGTGTTGAATGTGTCCCCCAA | 136 | AY496013 |
| R: CTCCCAAAGGGCAACAATGC | |||
| TFAM | F: GACTACTGCGTCTGCACCTT | 116 | NM_001130211 |
| R: GCAACTCTTCAGACCTCGCT | |||
| β-actin | F: TGCGGGACATCAAGGAGAAG | 216 | XM_003357928.2 |
| R: AGTTGAAGGTGGTCTCGTGG |
LAT1 = anti-L-type amino acid transporter 1; LAT4 = L-type amino acid transporter 4; SNAT2 = sodium-coupled neutral amino acid transporter 2; AMPKα = AMP-activated protein kinase α; PGC-1α = peroxisome proliferator-activated receptor gamma coactivator 1-alpha; SIRT1 = silent information regulator 1; Cyt c = cytochrome c; UCP2 = uncoupling protein 2; UCP3 = uncoupling protein 3; NRF-1 = nuclear respiratory factors-1; TFAM = transcription factor A.