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. 1999 Oct;121(2):479–488. doi: 10.1104/pp.121.2.479

Figure 1.

Figure 1

Sequences of the sad genes and the corresponding proteins. a, DNA sequences of the sadA, sadB, sadC, and MB3 cDNAs from pea cv Greenfeast. The start and stop codons are boxed, the MB3 primer sequences are underlined, and the nucleotides of the first primer matching the sad sequences are double underlined. Possible poly(A) signals (Hunt, 1994) are marked with a shaded area. b, Protein sequence alignment of the pea sadA, sadB, and sadC gene products with the translated sequences of the tomato gad3 gene (GenBank accession no. U21801; Jacobsen and Olszewski, 1996), the Arabidopsis Atsad2 gene product (GenBank accession no. AC004411; the second of four sad genes in the same genomic DNA region and by us named AtSad2), and Streptomyces exfoliatus (Marekov et al., 1990; SwissProt accession no. P19992). The catalytic YXXSK region is boxed, as well as the nucleotide binding site (Ghosh et al., 1991).