Table 2.
Primers for the real-time polymerase chain reaction (RT-PCR).
| Gene | ACCN | Primer sequence (5′ – 3′) | Product size, bp |
|---|---|---|---|
| β-actin | NM_205518 | F: ATCCGGACCCTCCATTGTC | 120 |
| R: AGCCATGCCAATCTCGTCTT | |||
| mTOR | XM_417614 | F: GGTGATGACCTTGCCAAACT | 220 |
| R: CTCTTGTCATCGCAACCTCA | |||
| S6K1 | NM_001030721 | F: CAATTTGCCTCCCTACCTCA | 176 |
| R: AAGGAGGTTCCACCTTTCGT | |||
| 4E-BP1 | XM_424384 | F: GCGAATGTAGGTGAAGAAGAG | 146 |
| R: AACAGGAAGGCACTCAAGG |
ACCN = accession number; mTOR = mammalian target of rapamycin; S6K1 = p70 ribosomal protein S6 kinase 1; 4E-BP1 = eukaryotic translation initiation factor 4E binding protein 1.