Table 2.
Parameters of primer pairs for IL-10, IL-22, IL-25, GAPDH genes of pigs.
| Gene | Genbank accession No. | Primer sequence (5′ to 3′) | Product, bp |
|---|---|---|---|
| IL-10 | NM_214041.1 | F: CTGACTGCCTCCCACTTTCT R: TTTGGGGAATGAGGTCAGGG |
150 |
| IL-22 | XM_001926156.1 | F: AACTTCCAGCAGCCCTACAT R: TGGGGAACAGCACTTCTTCA |
184 |
| IL-25 | XM_005666258.1 | F: GCCCCTTGGAGATACGAGTT R: CGGTAGAAGACGGTCTGGTT |
164 |
| GAPDH | NM_001206359.1 | F: ACATCATCCCTGCTTCTACTGG; R: CTCGGACGCCTGCTTCAC; |
187 |
IL-10 = interleukin-10; IL-22 = interleukin-22; IL-25 = interleukin-25; GAPDH = glyceraldehydes phosphate dehydrogenase.