Table 2.
Dietary composition and calculated nutrient specifications in starter (1–14 days post-hatch) and finisher (15–36 days post-hatch) experimental diets.
| Item | Starter1 |
Finisher1 |
||||||
|---|---|---|---|---|---|---|---|---|
| PC | NC1 | NC2 | NC3 | PC | NC1 | NC2 | NC3 | |
| Ingredient, g/kg | ||||||||
| Wheat | 637.7 | 654.7 | 666.7 | 678.7 | 741.6 | 750.8 | 762.8 | 771.1 |
| Soybean meal | 281.0 | 276.2 | 204.6 | 133.0 | 181.1 | 172.2 | 100.6 | 33.1 |
| Meat and bone meal | 0.0 | 0.0 | 60.0 | 120.0 | 0.0 | 0.0 | 60.0 | 120.0 |
| Vegetable oil | 41.6 | 37.1 | 36.1 | 35.1 | 44.1 | 39.5 | 38.5 | 37.5 |
| NaCl | 2.3 | 2.3 | 1.3 | 0.5 | 1.6 | 1.5 | 0.7 | 0.0 |
| Sodium bicarbonate | 2.0 | 2.0 | 2.0 | 1.6 | 1.8 | 2.0 | 1.7 | 1.0 |
| DL-methionine | 2.6 | 2.5 | 2.7 | 2.9 | 2.5 | 2.5 | 2.7 | 2.8 |
| Lysine HCl | 2.7 | 2.8 | 3.4 | 3.9 | 3.4 | 3.5 | 4.0 | 4.6 |
| Threonine | 1.1 | 1.1 | 1.3 | 1.6 | 1.6 | 1.7 | 1.9 | 2.2 |
| Limestone | 8.0 | 8.4 | 8.5 | 8.6 | 6.7 | 7.2 | 7.3 | 7.3 |
| Dicalcium phosphate | 15.4 | 7.3 | 8.0 | 8.6 | 10.0 | 1.6 | 2.3 | 2.9 |
| Choline chloride 60 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 |
| Vitamin–mineral premix2 | 5.0 | 5.0 | 5.0 | 5.0 | 5.0 | 2.0 | 2.0 | 2.0 |
| Celite | 0.0 | 0.0 | 0.0 | 0.0 | 15.0 | 15.0 | 15.0 | 15.0 |
| Nutrient level, g/kg | ||||||||
| Metabolisable energy, kcal/kg | 3,060.0 | 3,060.0 | 3,060.0 | 3,060.0 | 3,148.0 | 3,148.0 | 3,148.0 | 3,148.0 |
| Protein | 214.0 | 214.0 | 214.0 | 214.0 | 181.9 | 181.9 | 181.9 | 182.0 |
| Calcium | 9.0 | 7.2 | 7.2 | 7.2 | 7.0 | 5.2 | 5.2 | 5.2 |
| Total phosphorus | 7.3 | 5.6 | 5.3 | 5.0 | 5.8 | 4.2 | 3.9 | 3.6 |
| Phytate-P3 | 2.68 | 2.69 | 2.39 | 2.10 | 2.45 | 2.43 | 2.13 | 1.85 |
| Lysine | 12.5 | 12.5 | 12.5 | 12.5 | 10.5 | 10.5 | 10.5 | 10.5 |
| Methionine | 5.7 | 5.7 | 5.8 | 6.0 | 5.2 | 5.2 | 5.3 | 5.5 |
| Methionine + cysteine | 9.4 | 9.4 | 9.4 | 9.4 | 8.5 | 8.5 | 8.5 | 8.5 |
| Threonine | 8.5 | 8.5 | 8.5 | 8.5 | 7.6 | 7.6 | 7.6 | 7.6 |
| Sodium | 1.8 | 1.8 | 1.8 | 1.8 | 1.5 | 1.5 | 1.5 | 1.5 |
| Potassium | 8.5 | 8.4 | 7.3 | 6.2 | 6.8 | 6.7 | 5.6 | 4.4 |
| Chloride | 2.4 | 2.4 | 2.4 | 2.4 | 2.1 | 2.1 | 2.1 | 2.2 |
PC, positive control; NC1, negative control diet without MBM; NC2, negative control diet with 60 g/kg MBM; NC3, negative control diet with 120 g/kg MBM.
The vitamin–mineral premix supplied per tonne of feed: [MIU] retinol 12, cholecalciferol 5, [g] tocopherol 50, menadione 3, thiamine 3, riboflavin 9, pyridoxine 5, cobalamin 0.025, niacin 50, pantothenate 18, folate 2, biotin 0.2, copper 20, iron 40, manganese 110, cobalt 0.25, iodine 1, molybdenum 2, zinc 90, selenium 0.3.
Phytate-P calculation was based on 2.20 g/kg phytate-P in wheat and 4.53 g/kg phytate-P in soybean meal as reported in Selle et al. (2003).