TABLE 1 .
MICs for each variant included in the VIM-2 cluster
| Change introduced | Variant | MIC (µg/ml) ofa: |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| AMP | AMC | PTZ | CTX | CAZ | CEP | ERT | IMI | MER | ||
| None | VIM-2 | >256 | >256 | 48 | 16 | 12 | 0.5 | 0.75 | 1.5 | 0.75 |
| Q59R | VIM-36 | >256 | >256 | 24 | 12 | 64 | 0.125 | 0.19 | 2 | 1.5 |
| Y224H | VIM2Y224H | >256 | >256 | 48 | 24 | 12 | 0.25 | 0.19 | 1.5 | 0.38 |
| R228S | VIM-23 | >256 | >256 | 32 | 16 | 96 | 1.5 | 0.25 | 2 | 0.75 |
| R228L | VIM-24 | >256 | >256 | 24 | 8 | 128 | 1.5 | 0.047 | 1.5 | 1.5 |
| N165S | VIM-11 | >256 | >256 | 16 | 24 | 128 | 3 | 0.25 | 1 | 0.75 |
| H252R | VIM-20 | >256 | >256 | 8 | 6 | 24 | 0.25 | 0.094 | 0.75 | 0.25 |
| Y218F | VIM-15 | >256 | >256 | 64 | 12 | 12 | 0.75 | 0.094 | 2 | 1 |
| Q59R+R228S | VIM-2Q59R+R228S | 32 | 64 | 4 | 0.25 | 4 | 0.064 | 0.008 | 0.5 | 0.125 |
| Q59R+R228L | VIM-2Q59R+R228L | >256 | >256 | 12 | 1.5 | >256 | 0.5 | 0.25 | 1.5 | 0.38 |
| Q59R+N165S | VIM-6 | >256 | >256 | 24 | 2 | >256 | 0.5 | 0.19 | 2 | 1.5 |
| Q59R+H252R | VIM-2Q59R+H252R | >256 | >256 | >256 | 16 | >256 | 0.19 | 0.38 | 8 | 3 |
| Q59R+Y218F | VIM-2Q59R+Y218F | >256 | >256 | 48 | 4 | 32 | 0.25 | 1.5 | 6 | 3 |
| Y224H+H252R | VIM-31 | >256 | >256 | 64 | 16 | 24 | 1.5 | 1.5 | 4 | 0.75 |
| R228S+H252R | VIM-2R228S+H252R | 32 | 64 | 4 | 1.5 | 2 | 0.094 | 0.008 | 2 | 1.5 |
| R228S+Y218F | VIM-2R228S+Y218F | 32 | >256 | 1.5 | 4 | 3 | 0.25 | 0.016 | 0.75 | 0.38 |
| R228L+H252R | VIM-2R228L+H252R | >256 | >256 | 12 | 16 | >256 | 1 | 0.5 | 2 | 1.5 |
| R228L+Y218F | VIM-2R228L+Y218F | >256 | >256 | 12 | 12 | 24 | 8 | 0.5 | 3 | 0.75 |
| R228L+N165S | VIM-50 | >256 | >256 | 12 | 12 | >256 | 2 | 0.047 | 1.5 | 1.5 |
| H252R+Y218F | VIM-2H252R+Y218F | >256 | >256 | 16 | 24 | 24 | 1 | 0.75 | 4 | 0.75 |
| R228S+H252R+Y218F | VIM-2R228S+H252R+Y218F | 3 | 4 | 2 | 0.25 | 0.38 | 0.047 | 0.004 | 0.02 | 0.02 |
| R228L+H252R+Y218F | VIM-2R228L+H252R+Y218F | 4 | 4 | 2 | 0.4 | 0.4 | 0.094 | 0.007 | 0.19 | 0.032 |
a
The MICs are the medians of the 3 or more values obtained or a MIC value obtained more than once. AMP, ampicillin; AMC, amoxicillin-clavulanate; PTZ, piperacillin-tazobactam; CTX, cefotaxime; CAZ, ceftazidime; CEP, cefepime; ERT, ertapenem; IMI, imipenem; MER, meropenem.