Table 4.

Effects of spermine on CAT, T-AOC, and T-SOD activities and on GSH content of rat spleen.¹

Item	Spermine, μmol/g BW	Diquat, mg/kg BW	CAT, U/mg protein	GSH, mg/g protein	T-SOD, U/mg protein	T-AOC, U/mg protein
Group 1	0	0	11.8 ± 0.58	22.49 ± 0.52	13.69 ± 0.28	1.00 ± 0.02b
Group 2	0	12	9.39 ± 0.64	19.96 ± 0.51	11.89 ± 0.26	0.98 ± 0.02b
Group 3	0.4	0	16.4 ± 0.92	23.65 ± 0.52	13.46 ± 0.25	1.08 ± 0.02a
Group 4	0.4	12	13.0 ± 0.69	19.37 ± 0.53	12.41 ± 0.34	0.96 ± 0.02b
Main effects
Spermine	0		10.6 ± 0.51b	21.23 ± 0.38b	12.79 ± 0.20	0.99 ± 0.01
	0.4		14.7 ± 0.51a	21.51 ± 0.36a	12.93 ± 0.20	1.02 ± 0.01
Diquat		0	14.1 ± 0.51a	23.07 ± 0.36	13.58 ± 0.20a	1.04 ± 0.01
		12	11.2 ± 0.51b	19.67 ± 0.38	12.15 ± 0.20b	0.97 ± 0.01
P-value
Spermine			0.000	0.000	0.623	0.217
Diquat			0.000	0.597	0.000	0.001
Spermine × diquat			0.504	0.108	0.200	0.021

CAT = catalase; GSH = glutathione; T-SOD = total superoxide dismutase; T-AOC = total antioxidant capacity.

^a–bWithin a column, means with different superscript letters significantly differ (P < 0.05) for comparison between groups (Group 1 = control, Group 2 = diquat, Group 3 = spermine, Group 4 = spermine + diquat), between 2 doses of spermine (0 and 0.4 μmol/g BW, main effects), and between 2 doses of diquat injection (0 and 12 mg/kg BW, main effects) for one of all parameters (CAT, T-AOC, T-SOD, GSH), respectively.

¹Data are expressed as means ± SEM.