Table 2.
Oligonucleotide primers used for quantitative real time PCR.
| Target | Primer sequence (5′ to 3′)1 | Annealing temperature, °C | PCR product size, bp | GenBank accession No. |
|---|---|---|---|---|
| GAPDH | F: GCTGAATGGGAAGCTTACTG R: AAGGTGGAGGAATGGCTG |
60 | 216 | NM_204305.1 |
| IFN-γ | F: AGCTCCCGATGAACGAC R: CAGGAGGTCATAAGATGCCA |
62 | 151 | NM_205149.1 |
| TGF-β4 | F: GGACGGATGAGAAGAACTG R: ACGGACCACCATATTGGA |
62 | 296 | M31160.1 |
| IL-18 | F: GTTGTTCGATTTAGGGAAGGAG R: TCAAAGGCCAAGAACATTCC |
60 | 146 | NM_204608.1 |
| IL-10 | F: GACCAGCACCAGTCATCAG R: CCGTTCTCATCCATCTTCTCG |
62 | 159 | EF554720.1 |
| IL-2 | F: AGTCTTACGGGTCTAAATCACAC R: GGACAGCAGATTAGTTAGCCA |
62 | 219 | AF000631.1 |
| iNOS | F: AAAGAAAGGGATCAAAGGTGGT R: CAAGCATCCTCTTCAAAGTCTG |
60 | 296 | NM_204961.1 |
GAPDH = glyceraldehyde 3-phosphate dehydrogenase; IFN-γ = interferon-γ; TGF-β4 = transforming growth factor beta 4; IL = Interleukin; iNOS = inducible nitric oxide synthase.
1
F: Forward, R: Reverse.