Figure 4.

Intrinsic dynamics of the LeuT superfamily explains the structural variability observed experimentally for 104 conformers. Projections of the 104 conformers (electronic supplementary material, Table S1) onto ANM1 and the combined mode from PC2 and PC6 yielded a strong correlation (r = 0.82), revealing that the observed differences between these structures comply with the softest mode intrinsically encoded by the LeuT-fold. Colours and shapes represent different families and conformational states, respectively, as described in the legend. Three representative LeuT structures are displayed: outward-facing open (PDB: 3TT1), outward-facing closed (PDB: 2A65) and inward-facing open (PDB: 3TT3), with TM1, 6 and 10 coloured dark blue, orange and pink, respectively.