Table 1.
Inter-ring distances between main and side chain atoms of helix D in various high-resolution structures of GroEL.
| entry | PDB code | method | resolution | distance (Å) G88(N)–G88(N) | distance (Å) A106(Cα)– A106(Cα) | distance (Å) K105(ɛN)–A109(O) | |
|---|---|---|---|---|---|---|---|
| 1 | apo-wild-type | 1XCK | x-ray | 2.92 | 63.42 ± 0.12 n = 7 |
9.10 ± 0.14 n = 7 |
5.45 ± 0.27 n = 14 |
| 2 | apo-K105A | 4WSC | x-ray | 3.04 | 63.42 ± 0.21 n = 7 |
8.80 ± 0.21 n = 7 |
not measurable |
| 3 | apo-D83A/R197A | 4WGL | x-ray | 3.13 | 63.34 ± 0.26 n = 7 |
8.77 ± 0.13 n = 7 |
4.47 ± 0.45 n = 14 |
| 4 | ADP14 D83A/R197A | 4KI8 | x-ray | 2.72 | 62.46 ± 0.42 n = 7 |
9.06 ± 0.10 n = 7 |
4.13 ± 0.52 n = 14 |
| 5 | apo-wild-type | 5WOS | cryo-EM | 3.5 | 63.23 ± 0.30 n = 3 |
8.71 ± 0.72 n = 3 |
4.90 ± 0.10 n = 3 |
| 6 | ATP14 wild-type | 4AB2–4AAU | cryo-EM | 8–9 | 66.42 ± 1.33 n = 6 |
13.10 ± 1.50 n = 6 |
|
| 7 | ‘bullet’ ADP7 (cis) | 1AON | x-ray | 3.0 | 63.31 ± 0.04 n = 7 |
9.73 ± 0.04 n = 7 |
5.84 ± 0.05 (cis) 10.02 ± 0.10 (trans) |
| 8 | ‘football’ (ADP-BeF3)14 | 4PKN | x-ray | 3.60 | 67.23 ± 0.17 n = 7 |
12.67 ± 0.07 n = 7 |
7.98 ± 1.17 n = 14 |
| 9 | ‘football’ (ADP-BeFx)14 | 4PKO | x-ray | 3.84 | 66.74 ± 0.28 n = 7 |
12.38 ± 0.02 n = 7 |
7.88 ± 1.38 n = 14 |
| 10 | ‘football’ D52A/D358A (ATP)14 | 3WVL | x-ray | 3.79 | 66.55 ± 0.23 n = 7 |
12.36 ± 0.08 n = 7 |
9.08 ± 1.21 n = 14 |