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. 2018 May 7;373(1749):20170192. doi: 10.1098/rstb.2017.0192

Figure 3.

Figure 3.

Screening WD40-repeat proteins for CCT interaction. (a) 6% native PAGE analysis of TAF5, UTP13, ELP2 and DOA1 after in vitro translation in E. coli lysate (E) or rabbit reticulocyte lysate (R) in vitro transcription/translation system to detect GroEL (filled arrowhead) or CCT interaction (open arrowhead) respectively. (b) Graphs of time courses of CCT-bound protein by 6% native PAGE analysis of rabbit reticulocyte in vitro translation assays of TAF5, UTP13 and HAT2. (c) 6% native PAGE analysis of time course of rabbit reticulocyte in vitro translation assay of VID27. Inset image of sample at the end of the time course showing interaction of CCT with Vid27p which can be shifted with the anti-TCP1 antibody 23C, demonstrating specificity of CCT binding. Vid27p is the most avid CCT binding protein in yeast [19,25,46]. Vid27 is a little studied, non-essential, WD40-repeat protein most closely related to BUB3 and possibly involved in vacuolar protein degradation and is found in yeasts and plants but not in higher eukaryotes.