Fig. 4.

[³H]LSN3172176 association and dissociation kinetics. For association rate experiments (A) specific binding was calculated by subtracting the mean nonspecific binding in the presence of 10 μM atropine from the mean total binding at each time point performed in quadruplicate. For dissociation rate experiments (B) binding was expressed as a percent of total bound DPM (disintegrations per minute) at 0 minutes. Data points represent mean ± S.E.M. from three independent experiments each containing four replicates.