Fig. 7.

Native tissue saturation binding. Saturation binding experiments using [³H]LSN3172176 were conducted using brain membranes (frontal cortex and cerebellum) prepared from wild-type and M1 mAChR KO mouse, rat, rhesus monkey, and human. Specific binding was calculated by subtracting the mean nonspecific binding in the presence of 10 μM atropine from the mean total binding at each radioligand concentration performed in quadruplicate and then expressed as femtomole [³H]3172176 binding per milligram protein. Data points represent mean ± S.E.M. from three independent experiments.