TABLE 4.

Summary of native tissue K_d and B_max values for [³H]LSN3172176 binding across species and brain regions

Mean K_d and B_max values +/− S.E.M. were calculated from data presented in Fig. 7.

Tissue	3H-LSN3172176 Binding to Native Tissues
	Kd ± S.E.M.	Bmax ± S.E.M. Protein
	nM	fmol/mg protein
Mouse cortex	1.02 ± 0.15	839 ± 37
Mouse cerebellum	NSSB	NSSB
M1 KO mouse	0.1 ± 0.02	182 ± 6.5
Rat cortex	2.66 ± 0.89	562 ± 58
Rat cerebellum	NSSB	NSSB
Rhesus cortex	8 ± 3	843 ± 69
Rhesus cerebellum	NSSB	NSSB
Human cortex	1.03 ± 0.16	224 ± 35
Human cerebellum	NSSB	NSSB

NSSB, no significant saturable binding.