Fig. 3.

Effects of tested indoles on TCDD-inducible transcriptional activity of AhR. AZ-AHR cells were incubated for 8 and 24 hours with vehicle (DMSO; 0.1% v/v) and tested compounds in concentrations ranging from 100 nM to 200 μM. Incubations were carried out in the presence of TCDD (5 nM). Cells were lysed and luciferase activity was measured. Experiments were performed in four consecutive passages of AZ-AHR cells and the representative experiment is shown. Data are expressed as a percentage of the activation attained by 5 nM TCDD and are the mean ± S.D. from measurements performed in quadruplicate. *P < 0.05 indicates a value different from TCDD-treated cells.