Table 2.
Primers used for measurement of gene expression level by quantitative PCR.
| Gene description | Gene symbol | Primer sequences | qPCR efficiency, % | Amplicon size, bp | Annealing temperature, °C | Fluorescence acquisition temperature, °C | GeneCards identifiers |
|---|---|---|---|---|---|---|---|
| Actin, beta | ACTB | Up 5′ -CACGCCATCCTGCGTCTGGA- 3′ Low 5′ -AGCACCGTGTTGGCGTAGAG- 3′ |
93 | 452 | 58.7 | 72 | GC07M005566 |
| Glyceraldehyde-3-phosphate dehydrogenase | GAPDH | Up 5′ -ACACTCACTCTTCTACCTTTG- 3′ Low 5′ -CAAATTCATTGTCGTACCAG- 3′ |
95 | 90 | 49.3 | 81 | GC12P006643 |
| Ribosomal protein L4 | RPL4 | Up 5′ -CAAGAGTAACTACAACCTTC- 3′ Low 5′ -GAACTCTACGATGAATCTTC- 3′ |
94 | 635 | 60.0 | 72 | GC15M066790 |
| Metallothionein | MT1 | Up 5′ -TTGCTCTCTGCTTGGTCTCACCT -3′ Low 5′- GGGATGTAGCATGAAGTCAGTGCATGTG -3′ |
98 | 378 | 60.3 | 72 | NM 001001266 |
| Tumour necrosis factor-α | TNF-α | Up 5′- GCCCACGTTGTAGCCAATGTCAAA -3′ Low 5′- TTGTCTTTCAGCTTCACGCCGTTG -3′ |
99 | 98 | 58.0 | 72 | GC06P031543 |
| Nitric oxide synthase 2, inducible | NOS2 | Up 5′- TCCAGAAGCAGAACGTGACCATCA -3′ Low 5′- GAGCACGGCTTTGACCAAGACTTT -3′ |
93 | 293 | 58.0 | 72 | GC17M026083 |