Fig. 3. Identification of SRCIN1 as a direct target gene of miR-181a.

a Sequence pairing between mature miR-181a and the human SRCIN1 3′-UTR. All nucleotides of the seed sequence of the binding site are conserved in several species, including Homo sapiens, Pan troglodytes, and Oryctolagus cuniculus. The predicted free energy values of the hybrids are indicated. b, c Western blot analysis of SRCIN1 protein expression levels in the same paired CRC and normal tissue samples (n = 14); b representative images; c quantitative analysis. d Pearson’s correlation scatter plot of the fold changes in miR-181a and SRCIN1 protein expression in human CRC tissue pairs. e Clinical specimens of CRC and normal tissue samples were stained for miR-181a, SRCIN1. Representative images from a tissue microarray are shown, scale bars: 100 μm. ***P < 0.001