Table 3.

Growth performances and survival rate of juvenile olive flounder fed the experimental diets for 6 weeks.

Item1	WG,2 %	SGR,3 %/day	FE,4 %	PER5	FI6	Survival, %
Individual treatment means
Hg0E0	31.2 ± 8.0	0.99 ± 0.1b	60.8 ± 0.7bc	1.23 ± 0.1b	3.5 ± 0.4	96.7 ± 2.9
Hg0E100	34.3 ± 2.6	0.94 ± 0.1b	58.0 ± 0.9bc	1.15 ± 0.1b	3.7 ± 0.1	100 ± 0.0
Hg0E200	41.6 ± 5.6	1.12 ± 0.1a	70.5 ± 8.5a	1.44 ± 0.2a	3.8 ± 0.1	98.3 ± 2.9
Hg20E0	19.6 ± 3.1	0.58 ± 0.1c	35.7 ± 6.8d	0.71 ± 0.1c	3.7 ± 0.3	96.7 ± 2.9
Hg20E100	30.0 ± 4.4	0.92 ± 0.1b	55.1 ± 3.1c	1.10 ± 0.1b	3.8 ± 0.1	96.7 ± 2.9
Hg20E200	36.0 ± 3.1	0.98 ± 0.1b	64.4 ± 8.7ab	1.20 ± 0.1b	3.7 ± 0.1	98.3 ± 2.9
Means of main effect
Hg0	35.7a	1.0a	63.1a	1.3a	3.6	98.3
Hg20	28.5b	0.8b	51.7b	1.0b	3.7	97.2
E0	25.4c	0.8c	48.3c	0.9c	3.5	96.6
E100	32.1b	0.9b	56.5b	1.1b	3.8	98.3
E200	38.8a	1.1a	67.5a	1.3a	3.8	98.3
Two-way ANOVA: P-value
Hg effect	0.0085	0.0001	0.0004	0.0002	0.3753	0.3887
Vitamin E effect	0.0017	0.0001	0.0001	0.0003	0.1519	0.4719
Hg × Vitamin E	0.4151	0.0006	0.0033	0.0068	0.4716	0.4719

WG = weight gain; SGR = specific growth rate; FE = feed efficiency; PER = protein efficiency ratio; FI = feed intake.

^a,b,c Values are means ± SD from triplicate groups of fish (n = 3) where the values within a column without a common superscript differ (P < 0.05).

¹Hg₀E₀, Hg₀E₁₀₀ and Hg₀E₂₀₀ designate 0 mg/kg Hg with 0, 100 and 200 mg/kg vitamin E, respectively; Hg₂₀E₀, Hg₂₀E₁₀₀ and Hg₂₀E₂₀₀ designate 20 mg/kg Hg with 0, 100 and 200 mg/kg vitamin E, respectively.

²WG (%) = 100 × (Final weight–Initial weight)/Initial weight.

³SGR (%/day) = 100 × (ln Final weight–ln Initial weight)/Days.

⁴FE (%) = 100 × Wet weight gain (g)/Dry feed intake (g).

⁵PER = Wet weight gain/Protein intake.

⁶FI = 100 × Total feed fed (dry matter)/[(Initial weight + Final weight + Dead fish weight)/2 × Days].