Table 5.
Tissue total mercury contents of juvenile olive flounder fed the experimental diets for 6 weeks (μg/g of wet matter basis).
Item1 | Muscle | Kidney | Liver |
---|---|---|---|
Individual treatment means | |||
Hg0E0 | 0.08 ± 0.01 | 1.6 ± 0.7c | 1.2 ± 0.2c |
Hg0E100 | 0.07 ± 0.01 | 1.6 ± 0.4c | 1.6 ± 0.3c |
Hg0E200 | 0.08 ± 0.02 | 2.1 ± 1.4c | 1.7 ± 1.0c |
Hg20E0 | 0.4 ± 0.2 | 35.9 ± 11.9a | 35.1 ± 0.7a |
Hg20E100 | 0.3 ± 0.1 | 22.6 ± 0.6b | 18.7 ± 0.7b |
Hg20E200 | 0.2 ± 0.1 | 19.6 ± 6.3b | 22.1 ± 6.9b |
Means of main effect | |||
Hg0 | 0.1b | 1.8b | 1.5b |
Hg20 | 0.3a | 25.6a | 25.2a |
E0 | 0.3a | 18.2a | 18.1a |
E100 | 0.2ab | 12.1b | 11.9b |
E200 | 0.1b | 10.8b | 10.2b |
Two-way ANOVA: P-value | |||
Hg effect | 0.0002 | 0.0001 | 0.0001 |
Vitamin E effect | 0.0527 | 0.0318 | 0.0059 |
Hg × Vitamin E | 0.0618 | 0.0241 | 0.0458 |
a,b,c Values are means ± SD from triplicate groups of fish (n = 3) where the values within a column without a common superscript differ (P < 0.05).
Hg0E0, Hg0E100 and Hg0E200 designate 0 mg/kg Hg with 0, 100 and 200 mg/kg vitamin E, respectively; Hg20E0, Hg20E100 and Hg20E200 designate 20 mg/kg Hg with 0, 100 and 200 mg/kg vitamin E, respectively.