Fig. 2. Alkyne–sulfur distance screening and kinetics of palladium-mediated decaging. (a) Decaging reaction of substituted thioether propargyl carbamate protected 7-amino-4-methyl coumarin 1, 6, 7, 11–13 using Pd(COD)Cl₂10. (b) Increase in fluorescence of decaging reactions over time. Propargyl carbamate protected fluorophore has quenched fluorescence which is restored upon decaging. The reactions were performed at 100 μM final concentration of the fluorophores, with 10 equiv. of Pd(COD)Cl₂10. Data was normalized with respect to 100 μM of free fluorophore plus the final concentration of the palladium complex 10 (1 mM). (c) Kinetics of decaging of a PEGylated fluorophore. This was determined under pseudo-first order conditions using a 100 μM final concentration of the fluorophore and 10–40 equiv. of Pd(COD)Cl₂10.