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. Author manuscript; available in PMC: 2018 May 9.
Published in final edited form as: Methods Enzymol. 2017 Aug 21;595:303–329. doi: 10.1016/bs.mie.2017.07.007

Table 4.

Parameters for LC-MS/MS analysis of reaction products

Retention Time (min) Parent Ion Fragmentor Energy (V) Product Ion 1 Collision Energy 1 Product Ion 2 Collision Energy 2
MeTrp 6.6 202.0 130 160.1 10 132.0 21
Trp 6.2 188 130 146.1 10 118 21
SAM 0.8 399.1 90 250.1 14
SAH 1.4 385.4 136.0 100 24 134.0 24
5′-dA 2.8 252.1 136.0 90 13 119.0 50
Tyr (IS) 1.7 182.0 165.0 60 3 123.0 15