Figure 2.

SIRT1 attenuates inflammation induced by bleomycin in mouse lung. (A and B) Cells were counted and total protein was determined in BAL fluid (BALF). (C) RT-PCR was used to quantify Il-6, Il-1β, nitric oxide synthase (iNOS), and matrix metallopeptidase 9 (Mmp-9) mRNA abundance in lung tissues. mRNA levels were calculated relative to Gapdh. IL-1β (D) and IL-6 (E) BALF levels and IL-6 (F) and IL-1β (G) plasma levels were determined by ELISA. Mean (±SEM); *P < 0.05, **P < 0.001 versus mice treated with BLM plus vehicle. For each group, n = 6.