Figure 5.

SIRT1 reverses bleomycin-induced pulmonary fibrosis. (A) Mice received BLM and then Res beginning 10 days after bleomycin treatment. Lung inflammation and fibrosis were evaluated by H&E and Masson’s trichrome staining. Magnification, ×400. (B) Lung collagen, measured by Sircol assay (n = 4 mice per group). (C) Lung collagen mRNA was quantified by real-time PCR. mRNA levels were normalized to Gapdh. (B and C) Mean (±SEM); *P < 0.05, **P < 0.001 versus mice treated with BLM plus vehicle. n = 4 mice per group.