Figure 2.

Whole-blood cytokine levels after ex vivo stimulation with Toll-like receptor (TLR) agonists by rs832582 genotype. Whole blood was stimulated for 6 hours on 96-well plates coated with stimulating agents or media alone. Cytokine levels were measured by cytometric bead–based immunoassays, adjusted for monocyte counts, and log₂ transformed. Summary plots showing mean (±SD) cytokine levels in: (A) whole blood after stimulation with normal media based on rs832582 genotype (AG or AA: n = 328 versus GG: n = 18); (B) whole blood after stimulation with Pam3CysSerLys4 (Pam3CSK4) (TLR1/2 agonist) based on rs832582 genotype (AG or AA: n = 328 versus GG: n = 18); (C) whole blood after stimulation with Yersinia pestis LPS (TLR4 agonist) based on rs832582 genotype (AG or AA: n = 169 versus GG: n = 10); and (D) whole blood after stimulation with R848 (TLR7/8) based on rs832582 genotype (AG or AA: n = 169 versus GG: n = 10). P values for all comparisons were generated using the unpaired Student’s t test, and significant comparisons are designated with an asterisk. MCP-1 = monocyte chemoattractant protein-1.