Figure 3.

Luciferase reporter activity for p53 (n = 3) (A), NF-κB (n = 3) (B), and activator protein 1 (AP-1) (n = 4) (C) in human embryonic kidney cells 293 (HEK-293) are not influenced by coding variants in MAP3K1 SNP rs832582. All samples were processed in triplicate. The experimental output was relative luminescence units, which were derived by dividing firefly luciferase luminescence for each reporter construct by Renilla (control) luminescence at 48 hours after transfection with a MAP3K1 construct. Data are shown with each construct normalized to the empty vector value. P values for all pair-wise comparisons were generated using the Mann–Whitney test on normalized data.