Fig. 5.

Effect on HCC tumor ex vivo models. a Huh7 xenograft tumors from Balb/c nude mice (n = 5) were harvested and cut into small pieces of ~ 2 mm, and each piece was maintained in culture media. Tumor spheroids cultured from the pieces were treated with LAC117 (100 μg/mL) for 7 days. b Tumor spheroids were excised and processed for immunostaining for PCNA, cleaved caspase-3, and H&E staining. The results were observed by microscopy at ;× 400 magnification