Fig. 2.

Influence of G-CSF mobilization on cell composition. Peripheral blood of donors before and after mobilization, and leukapheresis products from G-CSF mobilized donors after Ficoll and cryopreservation were analyzed. A Flow cytometry was used to determine absolute cell counts of monocytes (mono.), granulocytes (gran.), lymphocytes (lymph.), and T cells per µl of peripheral blood before (“b”) and after (“a”) G-CSF mobilization in stem-cell donors, n = 6. B Relative subset composition of leukocytes in donors before and after G-CSF mobilization (n = 6). C Absolute cell count of CD45+ and CD3+ cells in stem cell grafts. The mean value ± SD of 15 preparations from 15 donors is shown. D Cell composition of leukocytes before and after mobilization in peripheral blood and after cryopreservation at day 0 before peptide stimulation. The proportion of T cells, B cells, NK cells, monocytes, and remaining unassigned leukocytes is shown at the three different time points, n = 5. A, B Statistical analysis was performed using the Mann–Whitney U test