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. 2018 May 9;16:124. doi: 10.1186/s12967-018-1498-3

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Comparison of cell composition on day 0 before peptide stimulation and day 9 at the end of expansion. a Relative composition of leukocytes at day 0 (d0) and day 9 (d9) after peptide stimulation from G-CSF mobilized (upper panel, n = 8) and non-mobilized donors (lower panel, n = 8). b Proportion of CD3+ T cells in total cells. c Proportion of CD8+ and CD4+ T cells within the CD3+ population. d Absolute counts of CD3+ T cells and of CD8+ CD3+ T cells. e Expression of T-cell differentiation markers on day 0 and day 9 of culture from one exemplary donor; cm, central memory; eff/em, effector and effector memory. f Cumulative analysis of T-cell differentiation in cultures from 7 donors of each type (G-CSF mobilized and non-mobilized donors)