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. 2018 May 9;16:124. doi: 10.1186/s12967-018-1498-3

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Analysis of CMV- and EBV-specific T cells before and after peptide stimulation. a Proportion of CMV-specific (CMV epitopes, left row) and of EBV-specific T cells (EBV epitopes, right row) on day 9 compared to day 0 as determined by HLA/peptide multimer staining (left panel: mobilized donors, n = 8; right panel: non-mobilized donors, n = 8). The cumulative proportions of T cells obtained from stainings with each applicable (HLA-compatible) multimer is shown for each donor. b Flow cytometric analysis for HLA/peptide multimer binding cells (% of CD8+ T cells) of one representative donor. c Comparision of absolute cell count of CMV-specific (CMV epitopes, left row) and EBV-specific T cells (EBV epitopes, right row) on day 9 compared to day 0 as determined by HLA/peptide multimer staining (left panel: mobilized donors, n = 8; right panel: non-mobilized donors, n = 8). a, c D01 to D12 designate the donors, as listed in Table 2