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. 2018 May 9;16:124. doi: 10.1186/s12967-018-1498-3

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Activation and cytokine production of CMV- and EBV-specific T cells. a CD25 expression as activation marker is shown on day 0 and day 9 for CD4+ and CD8+ T cells of G-CSF mobilized (left panel) and non-mobilized (right panel) donors, analyzed by flow cytometry. b Secretion of the effector cytokine IFN-γ was analyzed by ELISpot assay after restimulation with CMV or EBV peptide pool. Shown is the number of spot-forming cells (SFC)/50,000 PBMC as mean ± SD from 5 independent donors. As a negative control, the irrelevant gp100 peptide ITDQVPFSV was used