Fig. 3.

Expression profiles of a set of ABA biosynthesis- and signal transduction-related genes in SlbZIP1-RNAi plants. a Expression profiles of SlbZIP1 in leaves of WT and SlbZIP1-RNAi lines. b-d Expression profiles of ABA biosynthesis genes SlNCED1 and SlNCED2 (b), ABA-dependent receptor genes SlPYLs (c), and ABA-responsive element binding factor (ABF) genes SlABF2 and SlABF4 (d) between WT and SlbZIP1-RNAi plants under normal and ABA-treated conditions. 8-week-old WT and transgenic plants were sprayed with 100 μM ABA solution, leaf samples were harvested before and 2 h post the ABA treatment. The relative expression levels were normalized to 1 in control plants (0 h). Bars represent the mean of three biological replicates ± SE. Asterisks indicate statistical significance (*0.01 < P < 0.05, **P < 0.01) between WT and transgenic plants, respectively