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. 1999 Oct;121(2):525–534. doi: 10.1104/pp.121.2.525

Figure 2.

Figure 2

Hydroxylapatite column chromatography of the ATP extract. A, Silver staining of a 6% (w/v) SDS-polyacrylamide gel. B, Immunoblotting of the same fractions using AS-170. Numbers on the top of gels indicate fraction numbers. After the application of the ATP extract shown in Figure 1B on a hydroxylapatite column, the adsorbed materials were eluted with a discontinuous gradient of 5 mm (fraction 1–5), 150 mm (fraction 6–11), and 300 mm (fraction 12–17) potassium phosphate buffer. The arrowheads and arrow indicate 170-kD myosin and the 120-kD degradation product of the 170-kD heavy chain, respectively.