Fig. 5.

Fold-change of conserved and classical miRNA proved to be essential for reproductive development in 337S. a and c qRT-PCR data. The transcript level of each gene was normalized to NN1. The fold change normalized ≥ 2 or ≤ 0.5 were represented by numerical value. Error bars indicated s.d. based on three biological replicates (**P < 0.01, Student’s t-test). b and d Transcriptome sequencing data. The expression value of all genes was normalized to NN1. The fold change normalized ≥ 2 or ≤ 0.5 were used for judging significant differentially expressed genes in sequencing data and represented by numerical value