Table II.
Rates of conversion of derivatives of d-Glc to l-AA
| Substrate | Concentration | Rate of l-AA Biosynthesisa | nb | Relative Rate of l-AA Biosynthesisc |
|---|---|---|---|---|
| mm | ||||
| d-Glc | 30 | 0.4 | 1 | 0.8 |
| l-Glc | 30 | 0.5 | 1 | 0.7 |
| 3-O-methyl-dbGlc | 30 | 0.5 ± 0.3 | 2 | 1.3 ± 0.1 |
| d-Man | 30 | 0.1 | 1 | 0.3 |
| Myoinositol | 30 | 0.7 | 1 | 1.2 |
| l-Sorbosone | 30 | 0.4 | 1 | 1.2 |
| l-Sorbosone | 15 | 0.4 | 1 | 1.2 |
| d-GuL | 30 | 0.3 ± 0.1 | 2 | 0.7 ± 0.1 |
| d-GuL | 15 | 0.5 | 1 | 0.9 |
| l-GuL | 30 | 4.7 ± 0.5 | 2 | 14.7 ± 1.6 |
| l-GuL | 15 | 3.8 ± 0.9 | 4 | 9.7 ± 1.2 |
| d-Glucuronolactone | 30 | 1.4 | 1 | 3.1 |
| d-Glucuronolactone | 15 | 1.6 | 3 | 2.9 ± 0.4 |
| d-Xylonolactone | 30 | 0.4 | 1 | 0.7 |
| d-Mannonolactone | 30 | 0.5 | 1 | 0.8 |
| d-Gluconic acid | 15 | 0.5 | 2 | 1.2 ± 0.2 |
| d-GlcUA | 15 | 0.2 | 1 | 0.4 |
| MeGlcUA | 30 | 4.1 | 1 | 7.6 |
| MeGlcUA | 15 | 4.5 ± 0.1 | 2 | 8.5 ± 0.3 |
| l-AA | 30 | 12.8 | 1 | 42.7 |
| d-Isoascorbic acid | 30 | 1.1 | 1 | 3.5 |
| DHA | 30 | 30.7 | 1 | 96.0 |
a
All rates of l-AA biosynthesis are expressed as nmol total l-AA (l-AA + DHA), synthesized per hour, per 400-μL aliquot of cell suspension culture (on average 48.7 mg fresh weight).
b
n, Number of incubations carried out.
c
Relative rates of l-AA biosynthesis for each incubation represent the rate of total l-AA biosynthesis compared to the rate of l-AA biosynthesis in the control culture for each experiment.