Fig. 5.

GUS expression in transgenic Arabidopsis under conditions of sucrose or temperature stress. For sucrose stress, Col-0 and mutant seedlings were grown in 0.5× MS medium without sucrose (0 mM) or with 90 mM sucrose. Arabidopsis seedlings were grown for 10 days after sowing at a light intensity of 100 μmol^− 2 s^− 1 under long-day conditions. For temperature stress, Col-0 and mutant plants were grown on soil for 12 days at 22 °C with light intensity of 100 μmol^− 2 s^− 1 under long-day conditions. They were transferred to incubators at either 10 °C (low temperature) or 34 °C (high temperature) with a light intensity of 100 μmol^− 2 s^− 1 and incubated for 19 h. GUS staining was as described in Fig. 4