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. 2018 May 9;22:12. doi: 10.1186/s40824-018-0121-2

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Preparation of hFDM and characterization of heparin-grafted hFDM (hFDM-hep). a hFDM morphology before and after decellularization; b Toluidine blue staining of hFDM and hFDM-hep; c Quantitative measurement of heparin content; d Compositional analysis of hFDM-hep using ATR-FTIR; e Identification of TGF-β1 immobilized on the hFDM and hFDM-hep, respectively via immunofluorescence of TGF-β1 (green). Scale bar is 200 μm